Sample Training Agenda - we can customize to suit your needs
Microscopic Examination of Activated Sludge
Half-Day Training Agenda
Why use Microscopy as a Process Monitoring Tool?
· Life in an activated sludge system
· PowerPoint #1 – Microbial Life at an Activated Sludge WWTP
Introduction to the Microscope
· Overview of names and functions of microscope components
· Safety and care of the microscope
Wet Mount Preparation
· Demonstration
o Hands-on – students prepare wet mounts
o students examine wet mounts
Activated Sludge Theory of Operation
· Characteristics of a good sludge
· Microbial indications of poor performance
· PowerPoint #2 – When Good Bugs go Bad
…………………………………… BREAK …………………………………………………………
Preparation of a Smear (first step in preparing a Gram stain), Preparation of an India ink slide
· Demonstration – making a smear and preparing an India ink wet mount
o Hands-on – Students prepare smears and India ink wet mounts
o Students examine India ink wet mounts
Identification and Interpretation of Filaments in Activated Sludge
· Characteristics used to identify filaments
· High filaments? – so what? Interpretation of filament findings
· PowerPoint #3 – Identification and Interpretation of Filaments in Activated Sludge
Performing a Gram stain
· Demonstration – performing a Gram stain
o Hands-on – Students perform Gram stain & set aside to dry
Comprehensive Microscopic Examination
· Integrating wet mount, India ink wet mount and Gram stain results
· Completion of Mixed Liquor Exam Form
o Hands-on – Students examine Gram stains and use information from Gram stain and previous activities to complete a comprehensive Mixed Liquor Exam Form
Half-Day Training Agenda
Why use Microscopy as a Process Monitoring Tool?
· Life in an activated sludge system
· PowerPoint #1 – Microbial Life at an Activated Sludge WWTP
Introduction to the Microscope
· Overview of names and functions of microscope components
· Safety and care of the microscope
Wet Mount Preparation
· Demonstration
o Hands-on – students prepare wet mounts
o students examine wet mounts
Activated Sludge Theory of Operation
· Characteristics of a good sludge
· Microbial indications of poor performance
· PowerPoint #2 – When Good Bugs go Bad
…………………………………… BREAK …………………………………………………………
Preparation of a Smear (first step in preparing a Gram stain), Preparation of an India ink slide
· Demonstration – making a smear and preparing an India ink wet mount
o Hands-on – Students prepare smears and India ink wet mounts
o Students examine India ink wet mounts
Identification and Interpretation of Filaments in Activated Sludge
· Characteristics used to identify filaments
· High filaments? – so what? Interpretation of filament findings
· PowerPoint #3 – Identification and Interpretation of Filaments in Activated Sludge
Performing a Gram stain
· Demonstration – performing a Gram stain
o Hands-on – Students perform Gram stain & set aside to dry
Comprehensive Microscopic Examination
· Integrating wet mount, India ink wet mount and Gram stain results
· Completion of Mixed Liquor Exam Form
o Hands-on – Students examine Gram stains and use information from Gram stain and previous activities to complete a comprehensive Mixed Liquor Exam Form